Appendix A.

Project Approval

A.1.

DBMS Project proposal submitted to the University of Portsmouth and to King�s College Hospital for assessment and approval.

A.2.

Original� SPE method development proposal submitted to Departmental Executive in April 2008

A.3.

Ethical Approval

A.4.

Research and Development Governance Approval

Appendix B.

COSHH and Risk Assessments

B.1.

COSHH Assessments

B.2.

Assay Risk Assessment

B.3.

Equipment Risk Assessment

Appendix C.

Equations

Appendix D.

Chemical Structures of Drugs and Metabolites

Appendix E.

Laboratory Documentation

E.1.

SPE procedure (SOP v 3.0)

E.2.

Method Validation Document

E.3.

Extract from Toxicology Unit Training Records

E.4.

LC-MS Maintenance Form

E.5.

SPE procedure using Aria software (SOP v4.0)

E.6.

Turboflow Outstanding Work List

E.7.

Turboflow Method Validation Document

E.8.

Turboflow Standard Operating Procedure (SOP� v5.0)

Appendix F.

LC-MS Data Files

F.1.

ToxID configuration file for SPE method

F.2.

ToxID Configuration file for Turboflow method

F.3.

SPE Mass Spectrometer Method

F.4.

Turboflow Mass Spectrometer Method

F.5.

Turboflow HPLC Method

F.6.

SPE HPLC method

F.7.

EZChrom and Xcalibur Sequence Import

F.7.i.

EZChrom and Xcalibur Sequence import (Excel file)

F.8.

Aria Sequence Import

F.8.i.

Aria Sequence Import (Excel file)

Appendix G.

Publications

G.1.

Poster presented at The International Association of Forensic Toxicologists, Geneva, September 2008