README file for Fontana et al_Results_Sex Differences in Zebrafish General Information: 1. Title of dataset. “Female adult zebrafish (Danio rerio) show higher levels of anxiety-like behavior than males, but do not differ in learning and memory capacity” DATASET – Raw data for all behavioral analysis. 2. Date of deposit. 01/10/2019 3. PI / Author (or equivalent) Barbara D. Fontana; Madeleine Cleal; Matthew O. Parker 4. Project Zebrafish (Danio rerio) are widely used as a translational model for human neuropsychiatric conditions. Many studies have not considered sex differences in their analyses. Here, we studied sex differences of adult zebrafish in two behavioral domains: Anxiety and Memory. To assess whether sex influences anxiety-like responses, we used two different behavioral protocols, the novel tank diving task and the light-dark test. To assess sex differences in learning and memory tasks, we explored two memory domains, short-term spatial memory (free movement pattern Y-maze task) and short-term fear memory (Pavlovian fear conditioning task). Although we did not find any significant difference in learning and memory tasks, female zebrafish showed robust increases in anxiety-like behavioral endpoints in both anxiety tests. Overall, our data suggest that zebrafish is a sensitive model to work with sex differences when modeling anxiety-related disorders and this should be an important factor to consider in different experimental designs. 5. Funding information. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001 at the University of Portsmouth, UK. MOP receives funding from the British Academy and the Foundation for Liver Research (UK). MC is supported by a Science Faculty Studentship from the University of Portsmouth. 6. Data collection information: i) Date/time period of collection 25/03/2019 ii) Geographic location of collection (if applicable) Sharing & Access (only relevant if sharing data) 7. State licence(s) for use. CC BY 8. Any restrictions. NA 9. Request for Access contact (if applicable). Request for more access contact: barbara.fontana@port.ac.uk or matthew.parker@port.ac.uk 10. Information on outputs/publications that cite or use the data. Female adult zebrafish (Danio rerio) show higher levels of anxiety-like behavior than males, but do not differ in learning and memory capacity. Data Overview: 11. List data files. Fontana_et_al._Results_Sex_Differences_in_Zebrafish_.xlsx 12. Explain any relationships between files. N/A 13. For each file: i) Description of content: This file contains all the behavioral data used to analyze sex differences in zebrafish. The first spreadsheet contains the data for light dark test (time in light and time in dark), novel tank diving response (distance traveled, and time spent in each zone) and Pavlovian fear conditioning (Baseline, probe and index). Meanwhile, the second spreadsheet contains all the data for the FMP Y-maze across time (6-time bins of 10 min each) and the animals’ frequency of choice (tetragram data). ii) If not embedded within the file(s) provide variable metadata, missing data data definitions etc. Sex column: 1 represents female animals and 2 represents male animals. Methodological Information: 14. Link to methodology description / or brief description of method. More details about the methodology used to collect the data, methods used, and analysis can be found in the full version of the original paper. 15. Describe any quality assurance procedures. NA 16. Any specific information required to interpret/use use the data e.g. specific instrumentation/software information. The data was analyzed using the IBM SPSS® Statistics and the graphics were created using the GraphPad Prism version 8.00.