README file for 'RNA-seq comparison of immortalised myoblasts from dystrophic and WT mice'.



General Information:

1. Title of dataset.



RNA-seq comparison of immortalised myoblasts from dystrophic and WT mice



2. Date of deposit.



20/09/18



3. PI / Author (or equivalent)



Prof. Darek Gorecki



4. Project 



Identification of early phenotypic changes in Duchenne muscular dystrophy.



5. Funding information.



Muscular Dystrophy Association USA (MDA294571), the EU Interreg IV (TC2N);

the Higher Committee for Education Development in Iraq,

The Polish Ministry of National Defense project "Kościuszko" no: 523/2017/DA. 



6. Data collection information:

i) Date/time period of collection

20/09/18



ii) Geographic location of collection (if applicable)

University of Portsmouth



Sharing & Access (only relevant if sharing data)

7. State licence(s) for use.

CC BY-NC-ND


8. Any restrictions. 

None


9. Request for Access contact (if applicable).



10. Information on outputs/publications that cite or use the data.

Róg et al (In Press) "Dystrophicmdxmouse myoblasts exhibit elevated ATP/UTP-evokedmetabotropic purinergic responses and alterations in calcium signalling", BBA - Molecular Basis of Disease





Data Overview:



For points 11-16 below: A full list of data files, description of their content, relationships between the files, and full list of methods used to generate and analyse the data can be found at https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-7287/. Data are currently embargoed but will be made available following publication of the manuscript. 



11. List data files.

12. Explain any relationships between files.

13. For each file:

i) Description of content

ii) If not embedded within the file(s) provide variable metadata, missing data data definitions etc.

iii) Details of any specialised formats or abbreviations



Methodological Information:

14. Link to methodology description / or brief description of method.

15. Describe any quality assurance procedures.

16. Any specific information required to interpret/use use the data e.g. specific instrumentation/software information.